A number of labor intensive and time-consuming techniques are available for RNA isolation, purification and quantification. Quantification of RNA samples is carried out by measuring their absorption at 260 nm, while the quality and integrity of RNA samples are commonly based on gel electrophoresis accompanied by ethidium bromide visualization (one
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Sanitization steps involve validation to display the aptitude of lowering and Keeping microbial contamination at acceptable degrees. Validation of thermal methods should consist of a heat distribution review to show that sanitization temperatures are accomplished all over the technique, such as the system of use place valves. Validation of chemical